Method and apparatus intended for the filtration on a membrane o

Details Method and apparatus intended for the filtration on a membrane o Method and apparatus intended for the filtration on a membrane o

1987-03-31

1990-06-05

Spear, Frank

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210232, 210651, B01D 1300

Patent

active

049311859

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to a device for filtering a liquid and collecting on a membrane elements in suspension in this liquid for analysis thereof.
An example of application of this method and this apparatus, in accordance with the invention, relates more particularly to the analysis of milk.
For example, when it is desired to count in milk spores of Clostridium Tyrobutyricum which adversely affect the quality of cheeses particularly of the Emmental type, a method is required responding to high requirements of sensitivity and speed.
A method perfected by the applicant and which has already been published consists in concentrating the spores by filtering the milk under pressure through a filtering membrane, and in counting the colonies obtained on this filtering membrane applied on the selective gelose medium, after sufficient incubation time. In its principle this method is not original, what is original are the precise conditions for such filtration allowing volumes of milk to be filtered very much greater than those which can be filtered using the usual methods.
A difficulty arises in positioning and recovering the filter, as well as in the filtration procedure which must comply with very strict criteria of filtered volume, convenience in use and absence of parasite contaminations which are not satisfied by existing methods.
Analytic filtration is usually carried out using devices operating by suction, guaranteeing the absence of contamination of the filter, and easy use adapted to the high rates required by the analysis.
In the case of liquids which are not readily filterable, such as milk, it is advantageous to increase the filtered volume to work under pressure, but the pressurized filtration apparatus available commercially, intended for the preparatory sterilization of the liquids, are not adapted to the repeated high rate use required by the analysis, and do not readily ensure the absence of parasite contamination of the filter.
The purpose of the present invention is precisely to describe a method and equipment for carrying out the analytic filtration operations under pressure under better conditions of convenience of use and recovery of the constituents to be analyzed.
The invention relates to the filtration of milk such as described above by way of example, but may also apply to the filtration of other liquids.
It relates more precisely to a method for filtering a product under pressure, on a filter or porous membrane, for recovering microorganisms on said filter, characterized in that it includes three essential cycles carried out using the same apparatus, namely: pressurization thereof, that the liquid introduced into the reservoir transits under pressure through the filter before being discharged. reservoir, the filter being still in position. therein.
The invention also relates to an apparatus for implementing the method, characterized in that it comprises a filter holder (membrane) whose positioning in particularly easy.
The invention will be better understood from the description of the apparatus and the operation thereof which follows, and from the accompanying Figures in which:
FIG. 1 shows the assembly of the filtration device, the system for locking the filter being in the operational position;
FIG. 2 is a top view of the filter holder;
FIG. 3 shows this system for locking the filter and filter holder, in the released position.
For the sake of clarity, the same elements bear the same references in all the Figures.
As shown in FIGS. 1 to 3, this apparatus is characterized in that it is formed of a case 2, shown schematically, which receives two active assemblies.
The first active assembly 100, disposed vertically on the upper part of the apparatus, includes a cylinder 3 serving as reservoir for the product to be filtered, with associated supply and pneumatic systems.
At the top of this reservoir 3 is located a sealed cover 4 fixed via an O seal 40, in which a pneumatic system 5 is fitted. A supply tube 6, associated with an electromagnetic assembly 7, opens into the reservoir cyli

REFERENCES:
patent: 2763308 (1956-09-01), Samler
patent: 2904857 (1959-09-01), Goetz
patent: 3448011 (1969-06-01), Russomanno
patent: 4094784 (1978-06-01), Hirano
patent: 4579662 (1986-04-01), Jonsson
patent: 4738782 (1988-04-01), Yamauchi et al.
Membranfiltration in der kosmetischen Industrie, Kosmetik und Aerosole, vol 99, #13, Jun. 14, 1973.

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