Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-03-15
2011-03-15
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S002000, C435S004000, C435S007720, C435S019000, C435S286100, C435S374000, C436S063000, C436S164000, C436S172000, C422S051000
Reexamination Certificate
active
07906295
ABSTRACT:
A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.
REFERENCES:
patent: 3586859 (1971-06-01), Katz et al.
patent: 4783401 (1988-11-01), Horan et al.
patent: 5436134 (1995-07-01), Haugland et al.
patent: 5437980 (1995-08-01), Haugland
patent: 5563070 (1996-10-01), Yamamoto et al.
patent: 5582984 (1996-12-01), Bieniarz et al.
patent: 5658751 (1997-08-01), Yue et al.
patent: 5939282 (1999-08-01), Harman et al.
patent: 6277655 (2001-08-01), Sarkadi et al.
patent: 6673568 (2004-01-01), Fleming et al.
patent: 7527924 (2009-05-01), Fleming et al.
patent: 2002/0119438 (2002-08-01), Kato
patent: WO 92/02632 (1992-02-01), None
Guldfeldt et al., Relationship between Yeast Cell Proliferation and Intracellular Esterase Activity during Fermentation, J. Inst. Brew., Nov. to Dec. 104: 333-338 (1998).
Breeuwer et al., Energy Dependent, Carrier-Mediated Extrusion of Carboxyfluorescein fromSaccharomyces cereviseaeAllows Rapid Assessment of Cell Viability by Flow Cytometry, Applied and Environmental Microbiology 60(5): 1467-1472 (May 1994).
Allen, F., “The Microbrewery Laboratory Manual—A Practical Guide to Laboratory Techniques and Quality Control Procedures for Small—Scale Brewers,”Brewing Techniques 2(4): 28-35, Jul./Aug. 1994.
Breeuwer et al., “Energy-Dependent, Carrier-Mediated Extrusion of Carboxyfluorescein fromSaccharomyces cerevisiaeAllows Rapid Assessmet of Cell Viability by Flow Cytometry,”Applied and Environmental Microbiology 60(5): 1467-1472, May 1994.
Breeuwer et al., “Characterization of Uptake and Hydrolysis of Fluorescein Diacetate and Carboxyfluorescein Diacetate by Intracellular Esterases inSaccharomyces cerevisiae, Which Result in Accumulation of Fluorescent Product,”Applied and Environmental Microbiology 61(4): 1614-1619, Apr. 1995.
Carvell et al., “Developments in Using Off-Line Radio Frequency Impedance Methods for Measuring the Viable Cell Concentration in the Brewery,”J. Am. Soc. Brew. Chem. 58(2): 57-62, 2000.
Catt et al., “Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human spermatozoa,”Molecular Human Reproduction 3(9): 821-825, 1997.
Ferguson and Denny, “Microbial mutagenic effects of the DNA minor groove binder pibenzimol (Hoechst 33258) and a series of mustard analogues,”Mutation Research 329: 19-27, 1995.
Griffith et al., “An evaluation of luminometry as a technique in food microbiology and a comparison of six commercially available luminometers,”Food Science and Technology Today 8(4): 209-216, Dec. 1994.
Guldfeldt et al., “Relationship Between Yeast Cell Proliferation and Intracellular Esterase Activity During Brewing Fermentations,”J. Inst. Brew. 104: 333-338, Nov.-Dec. 1998.
Kawai et al., “Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process,”J. App. Microbiology 86: 496-504, 1999.
Koch et al., “Fluorescence Microscopy Procedure for Quantitation of Yeasts in Beverages,”Applied and Environmental Microbiology 52(3): 599-601, Sep. 1986.
Kova{hacek over (r)}ík et al., “Estimation of viable cell count after fluorescein diacetate standing using phosphoimager analysis,”Biotechniques 27(4): 685-688, Oct. 1999.
Latt and Wohlleb, “Optical Studies of the Interaction of 33258 Hoechst with DNA, Chromatin, and Metaphase Chromosomes,”Chromosoma 52: 297-316, 1975.
Mochaba et al., “Practical Procedures to Measure Yeast Viability and Vitality Prior to Pitching,”J. Am. Soc. Brew. Chem. 56(1): 1-6, 1998.
O'Connor-Cox et al., “Methylene Blue Staining. Use At Your Own Risk,”Technical Quarterly 34(1): 306-312, 1997.
Prosperi, E., “Intracellular turnover of Fluorescein diacetate. Influence of membrane ionic on Fluorescein efflux,”Histochemical Journal 22: 227-233, 1990.
Smart et al., “Use of Methylene Violet Staining Procedures to Determine Yeast Viability and Vitality,”J. Am. Soc. Brew. Chem. 57(1): 18-23, 1992.
Steward et al., “A new methodology for plant cell viability assessment using intracellular esterase activity,”Plant Cell Reports 19(2): 171-176, Dec. 1999.
Fleming James E.
Holcomb Jerad R.
McLean Darby
Somes Jason Buck
Gabel Gailene R
GenPrime, Inc.
Seed IP Law Group PLLC
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