Method and apparatus for use in microscope investigations with a

Image analysis – Histogram processing – For setting a threshold

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Details

350531, 382 6, G02B 2126, G02B 2736

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active

050005545

ABSTRACT:
An assembly for use with an optical microscope in visually detecting the presence of a microscopic object in a sample and determining the position of the object in the sample to enable repeated visual observation of the object, the microscope having a microscope stage and an objective lens system with an optical path, comprises a slide holder removably attachable to the microscope stage in a plurality of alternative positions with respect to the stage for movably holding a slide bearing the sample, the slide holder including a frame stationary with respect to the stage and a slide carrier automatically shiftable with respect to the frame and the stage. The assembly further comprises a drive operatively connected to the slide holder for shifting the carrier and the slide carried thereby along a search path to enable a visual inspection of the sample. Mounting elements are provided on the carrier for mounting the slide to the carrier and for positioning the slide at a predeterminable fixed position and orientation with respect to the carrier. A monitoring device is operatively connected to the slide holder and to the drive for measuring changes in the position of the carrier relative to the frame and signaling the drive as to the measured changes in the position of the carrier. The carrier is provided with a zeroing mark for establishing an x-y coordinate system reference position of the carrier with respect to the frame, the zeroing mark being an x-y coordinate system reference mark viewable by an observer through the lens system upon a mounting of the slide holder to the microscope stage carrier.

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