Method and apparatus for two-dimensional measurement of concentr

Details Method and apparatus for two-dimensional measurement of concentr Method and apparatus for two-dimensional measurement of concentr

1991-12-18

1993-05-25

Fields, Carolyn E.

Radiant energy

Invisible radiant energy responsive electric signalling

Ultraviolet light responsive means

250372, G01J 142, G01N 2133

Patent

active

052142884

ABSTRACT:
A method of making a two-dimensional measurement of the concentration of a biological macromolecule aqueous solution and an apparatus for carrying out this method are disclosed. By charging the biological macromolecule aqueous solution and an aqueous crystallizing agent solution into a crystallizing cell, irradiating the crystallizing cell with an ultraviolet light from an ultraviolet light source, two-dimensionally scanning the ultraviolet light transmitted through the crystallizing cell by a linear image sensor to detect the quantity of the transmitted light, and carrying out a conversion of the detected quantity of the transmitted light to the protein concentration, the process of a crystallization of the biological macromolecule from biological macromolecule aqueous solution can be easily and accurately twodimensionally measured.

REFERENCES:
patent: 3751167 (1973-08-01), Claus
patent: 4192996 (1980-03-01), Kronick et al.
patent: 4207892 (1980-06-01), Binder
patent: 4587428 (1986-05-01), Binder
patent: 4919899 (1990-04-01), Herrmann et al.
P. J. Shlichta, "Feasibility of Mapping Solution Properties During the Growth of Protein Crystals," Journal of Crystal Growth, vol. 76, No. 3, Aug. 1986, Amsterdam, NL, pp. 656-662.

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