Method and apparatus for three-dimensional microscopy with enhan

Details Method and apparatus for three-dimensional microscopy with enhan Method and apparatus for three-dimensional microscopy with enhan

1995-02-03

1997-09-23

Nguyen, Thong

Optical: systems and elements

Compound lens system

Microscope

359368, 359370, 359383, G02B 2106, G02B 2100

Patent

active

056710852

ABSTRACT:
A method and apparatus for three dimensional optical microscopy is disclosed which employs dual opposing objective lenses about a sample and extended incoherent illumination to provide enhanced depth or Z direction resolution. In a first embodiment, observed light from both objective lenses are brought into coincidence on an image detector and caused to interfere thereon by optical path length adjustment. In a second embodiment, illuminating light from an extended incoherent light source is detected to the sample though both objective lenses and caused to interfere with a section of the sample by adjusting optical path lengths. Observed light from one objective lens is then recorded. In a third embodiment, which combines the first two embodiments, illuminating light from an extended incoherent light source is detected to the sample through both objective lenses and caused to interfere with a section of the sample by adjusting optical path lengths. The observed light from both lenses is caused to interfere on the image detector by the same optical path length adjustment.

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