Method and apparatus for thermal cycling and for automated sampl

Details Method and apparatus for thermal cycling and for automated sampl Method and apparatus for thermal cycling and for automated sampl

1996-08-27

1999-04-27

Jones, W. Gary

Chemical apparatus and process disinfecting, deodorizing, preser

Chemical reactor

Organic polymerization

422104, 422 64, 422 67, 422 99, 4352861, B01L 900, C12M 302

Patent

active

058978429

ABSTRACT:
An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reaction vessels, such as capillary tubes, for the preparation and thermal cycling of reaction mixtures. In order to prevent loss of the reaction mixture from the vessels during heating, the thermal cycling apparatus of the invention provides means for sealing the proximal and distal end of each reaction vessel. The proximal ends can be sealed by coupling to a pump which permits movement of the samples within the reaction vessels. As to the distal ends, the reaction vessels can be sealed by pressing the distal end of each vessel against a sealing element with a conformable surface, or by immersing the distal end of each vessel in the reservoir of liquid, preferably of an oil, that is not miscible with the reaction mixture.

REFERENCES:
patent: 5176203 (1993-01-01), Larzul
patent: 5451500 (1995-09-01), Stapleton
patent: 5455175 (1995-10-01), Wittwer et al.
patent: 5475610 (1995-12-01), Atwood et al.
patent: 5578270 (1996-11-01), Reichler et al.
patent: 5795547 (1998-08-01), Moser et al.
Advertisement for Pharmacia AutoLoad Solid Phase Sequencing Kit.
Brochure for Vistra DNA Labstation 625 (1994).
Advertisement for Qiagen BioRobot 9600.
Brochure for Beckman Biomek 2000 (1994).
Oste, C.C. "PCR Instrumentation: Where Do we Stand", Polymerase Chain Reaction, pp. 165-173 (1994).

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