Method and apparatus for the submerged growth of cell cultures

Details Method and apparatus for the submerged growth of cell cultures Method and apparatus for the submerged growth of cell cultures

1981-11-16

1988-06-07

Weimar, Elizabeth

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524025, 435286, 435818, 261122, 210150, 2103232, 2103216, C12N 502, C12M 302, B01D 2914, B01D 1300

Patent

active

047496541

ABSTRACT:
In a method and apparatus for the submersed growth of human and animal cell cultures, the cell growth container is provided with a centrally disposed permeator, which is formed simultaneously as conduit tube and reactor, and is provided on both sides with gas-permeable membranes. A gas of defined composition is led through the hollow porous support body of the permeator as fresh gas on one side, and led away on the other side as waste gas. In this manner the gas delivers oxygen across the membrane and receives carbon dioxide. The nutrient substrate and the tissue cells are held in suspension by means of a stirrer provided in the interior of the permeator. Foam problems do not occur, since no gas bubbles can enter into the medium.

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