Method and apparatus for the removal of non-uniformities in an e

Details Method and apparatus for the removal of non-uniformities in an e Method and apparatus for the removal of non-uniformities in an e

1997-03-10

1999-04-27

Warden, Robert

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204461, 356344, G01N 2726

Patent

active

058977600

ABSTRACT:
A method and apparatus for correcting non-uniformities in the source and the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity, correcting for source and lens non-uniformities allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. The non-uniformities due to the illumination source are characterized by sampling a portion of the source with a linear detector array and creating a correction data file. In order to sample the source, a mirror or beamsplitter is appropriately positioned, for example along the central optical axis of the electrophoresis apparatus. Similarly, a correction data file representing the non-uniformities due to the lens assembly is created using a secondary linear source of known uniformity. After the correction data files are stored, an image of the sample is taken and a sample data file is created. The sample data file can be normalized using the correction data files thereby creating a corrected sample file which can either be displayed or stored for future use.

REFERENCES:
patent: 4737464 (1988-04-01), McConnell et al.
patent: 4822168 (1989-04-01), Nogami et al.
patent: 4874492 (1989-10-01), Mackay
patent: 5294323 (1994-03-01), Togusari et al.
patent: 5307148 (1994-04-01), Kambara et al.
patent: 5672881 (1997-09-01), Striepeke et al.

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