Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1992-03-16
1999-01-26
Kumar, Shailendra
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530399, 530412, C07K 300
Patent
active
058640215
ABSTRACT:
Transforming growth factor .beta. (TGF-.beta.) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF-.beta. and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF-.beta. may be removed while the protein is bound to the cation exchange resin. Purified TGF-.beta.1 compositions have a specific activity above 10.sup.7 U/ml.
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Nestaas Eirik
Pungor, Jr. Erno
Berlex Laboratories Inc.
Kumar Shailendra
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