Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1989-09-11
1991-08-27
Raymond, Richard L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530412, 530416, C07K 322
Patent
active
050434314
ABSTRACT:
Transforming growth factor .beta. (TGF-.beta.) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF-.beta. and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF-.beta. may be removed while the protein is bound to the cation exchange resin.
REFERENCES:
patent: 4332717 (1982-06-01), Kanaoka et al.
patent: 4444760 (1984-04-01), Thomas, Jr.
patent: 4751078 (1988-06-01), Nagabhushan et al.
patent: 4894439 (1990-01-01), Dorin et al.
patent: 4897464 (1990-01-01), Valee et al.
patent: 4931548 (1990-06-01), Lucas et al.
Massaque (1984), J. Biol. Chem. 259: 9756-9761.
Van den Eignden-Van Raaij et al. (1989), Biochem. J. 257: 375-382.
Associan et al. (1983), J. Biol. Chem. 258: 7155-7160.
Frolik et al. (1983), Proc. Natl. Acad. Sci. USA 80: 3676-3680.
Roberts et al. (1983), Biochem. 22: 5692-5698.
Nestaas Eirik
Pungor, Jr. Erno
Codon
Raymond Richard L.
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