Method and apparatus for the on-location field detection of unid

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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422 56, 422 57, 422 58, 422 61, 435 792, 4352871, 4352872, 4352877, 4352881, 4352882, 4352885, 435810, 436169, 436514, 436518, 436524, 436528, 436535, 436805, 436810, 436172, G01N 3353

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058467459

ABSTRACT:
A method and test device are disclosed for detecting under field conditions unidentified antigenic substances which are detrimental to human or animal health or welfare. A sample solution containing the suspected unidentified antigenic substance is added to the test device containing a known antigenic substance which is irreversibly attached to a solid support and suspended in a buffer solution along with an antibody specific to the known antigenic substance conjugated with a detecting agent. After incubation to bind the unknown antigenic substance to the conjugate, the solution contacts a white porous bibulous sheet incorporating a substrate for the detecting agent and a chromogen, which is part of the test device. If the identity of the sample antigen is the same as that on the solid support, a color reaction takes place.

REFERENCES:
patent: 4275149 (1981-06-01), Litman et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 5156948 (1992-10-01), Christensen et al.
patent: 5234840 (1993-08-01), Appleton
patent: 5552288 (1996-09-01), Christensen et al.

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