Method and apparatus for the detection of pathogens,...

Chemistry: electrical and wave energy – Apparatus – Electrolytic

Reexamination Certificate

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C204S403010, C204S409000

Reexamination Certificate

active

07931788

ABSTRACT:
The present invention is directed to a method and apparatus for an immunoassay technique that uses amperometric measurements to rapidly analyze different pathogenic microorganisms, including bacteria, viruses, toxins, and parasites. In accordance with one aspect of the present invention, at least one conductive membrane is used to provide support for antibody immobilization and serve as a working electrode; it could also be independent rather than the working electrode. This conductive membrane or powder can be fabricated of a conductive material or can be a nonconductive material over which a conductive material is coated. In either case, the proposed technique is adaptable for use with different materials so as to form a membrane having a pore size that is suited to the particular application. Another aspect of the present invention relates to a compact and simple disposable element that can be easily disposed of after use. In still another aspect of the present invention, the immunoassay can be automated using microprocessor control so as to reduce the amount of human intervention in sample analysis.

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Shah et al. Rapid Amperometric Immunoassay forEscherichia coliBased on Graphite Coated Nylon Membranes, Electroanalysis, vol. 15 issue 23-24, 2003, pp. 1809-1814.

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