Method and apparatus for streaking a culture medium

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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435292, 435293, 435295, 435297, 435298, 435 30, C12M 126

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active

049818023

ABSTRACT:
This invention relates to an apparatus and method for streaking a sample on a Petri plate. The objective is to provide a method and apparatus in which the inoculum is sequentially streaked in a series of dilutions in a manner closely simulating previous manual practice but with a consistency, safety and accuracy which is not manually achieved. A sequential series of spreader heads are brought into contact with the culture medium on a Petri plate and swept back and forth over the medium surface to streak the inoculated sample in a segment of the plate. The paths of the second and subsequent spreading heads are angularly displaced from the path of the preceding spreading head, so that the segment of the plate in which the second and subsequent spreading heads streak the sample will partially overlap the respective preceding segment. This is best achieved by oscillating the support of the Petri plate and sequentially moving a spreading head radially during such oscillation. Provision is included for sterilizing the spreading head. Provision is also included for successively processing a series of Petri plates.

REFERENCES:
patent: 3623958 (1971-11-01), Fitzgerald
patent: 3935075 (1976-01-01), Perry et al.
patent: 4010077 (1977-03-01), Pardos
patent: 4144135 (1979-03-01), Sequeira
patent: 4287301 (1981-09-01), Astle

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