Method and apparatus for separation of high-molecular-weight sub

Imperforate bowl: centrifugal separators – With condition responsive means

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494 10, 494 36, 494 37, 494 84, B04B 1102

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049521276

ABSTRACT:
An improved method and apparatus for separating high-molecular-weight substances from cells suspended in liquid nutrient media, features continuous central monitoring and control of the process by a computer (22), and a way to combine the heretofore separate processes of first, separating media into solid and liquid components, and second, concentrating the proportion of high-molecular-weight components in the separated liquid. The media are conveyed by a first pump (3) to a centrifuge continuous flow rotor (2), which separates the solids from the liquid components and directs the latter to an intermediate reservoir (5), which sits on an electric scale (7) which is continuously monitored. From here they are fed to a filtration unit (9) in which the high-molecular-weight substances are separated and recycled through a feedback line (11), with the aid of a second pump (10), into the intermediate reservoir (5). The central computer and control unit (22) controls the centrifuge and pumps, monitors the scale (7) and pressure sensors (13), and adjusts the process parameters. An optical sensor (26) monitors the solids content at the centrifuge output (4).

REFERENCES:
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patent: 4261507 (1981-04-01), Baumler
patent: 4285810 (1981-08-01), Kirkland
patent: 4475897 (1984-10-01), Bradtmoller
patent: 4776834 (1988-10-01), Muller
Intel Microcontroller Handbook 1985, Title & Contents Pages and p. 7-1.

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