Method and apparatus for separating particles in liquid suspensi

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, 2041831, 2041821, G01N 2726

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active

049118064

ABSTRACT:
Small dielectric particles such as biological cells or DNA molecules are suspended in a quantity of liquid within a container. A pair of spaced apart electrodes are immersed in the liquid and a coil surrounds the region between the electrodes. First and second electric signals are applied to the electrodes and the coil, respectively, to thereby generate substantially orthogonal oscillating electric and magnetic fields which are at the same frequency but approximately ninety degrees out of phase. By selecting the frequency, particles having different polarization relaxation frequencies and sizes will migrate at different velocities and thereby sort into various fractions.

REFERENCES:
patent: 3207684 (1965-09-01), Dotts, Jr.
patent: 4326026 (1980-01-01), Sarkar
Pohl, Herbert A., "The Force on a Neutral Dielectric Object of Biological Interest in Crossed Time-Varying Electric and Magnetic Fields", Journal of Biological Physics 13(3), 1985, pp. 79-80.
"Magnetic Separation in Biotechnology", by Whitesides et al., 1983.
"Separation of Chromosome-Sized DNA Molecules by Pulsed Field Gel Electrophoresis", Van Der Ploeg, 1987.
"The Force on a Neutral Dielectric Object of Biological Interest . . . ", Pohl, Mar. 24, 1986.

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