Liquid purification or separation – Processes – Separating
Patent
1983-02-18
1984-12-11
Fisher, Richard V.
Liquid purification or separation
Processes
Separating
210516, 210927, 435 2, 436 63, 436177, B01D 2126
Patent
active
044877005
ABSTRACT:
The present invention relates to apparatus and method for separating, by centrifugation, blood lymphocytes from other leukocytes in a blood sample. Small dense particles, either wetted or suspended in a physiological, hydrophilic medium, and a chemically inert hydrophobic barrier material are initially located in a suitable container. When the blood sample is added to the container, the particles are phagocytized by the monocytes and granulocytes, so as to increase their specific gravities. Upon centrifugation, the barrier material, whose specific gravity is only slightly greater than the specific gravity of the lymphocytes and less than the respective specific gravities of the phagocytic cells which have ingested particles and of the erythrocytes (red blood cells) in the blood sample, forms a rigid barrier therebetween, allowing harvesting of the lymphocytes, substantially free of contamination.
REFERENCES:
patent: 3700555 (1972-10-01), Widmark et al.
patent: 3709791 (1973-01-01), Lichtenstein
patent: 4021340 (1977-05-01), Zine, Jr.
patent: 4190535 (1980-02-01), Luderer et al.
patent: 4250041 (1981-02-01), Babson et al.
patent: 4343793 (1982-08-01), Wissler
Czaja John W.
Fisher Richard V.
Herron C. J.
Technicon Instruments Corporation
Tedesco S. P.
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