Method and apparatus for selectively severing and analyzing...

Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...

Reexamination Certificate

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C436S164000

Reexamination Certificate

active

08030090

ABSTRACT:
A biological sample solution is ionized by electrospray and the sample solution is irradiated with an infrared laser beam to dissociate biological macromolecules into the constituents thereof. As a result, only non-covalent bonds of the biological macromolecules can be selectively severed and analyzed.

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Takamizawa et al. “Selective dissociation of non-covalent bonds in biological molecules by laser spray”, J. Mass Spectrom. 2004 v. 39, pp. 1053-1058.
Leisner et al. “Infrared Laser Post-Ionization of Large Biomolecules from an IR-MALD(I) Plume”, J Am Soc Mass Spectrom 2004, v. 15, pp. 934-941.
“Activation Energies for Dissociation of Double Strand Oligonucleotide Anions: Evidence for Watson—Crick Base Pairing in Vacuo,” Paul D. Schnier, John S. Klassen, Eric F. Strittmatter, and Evan R. Williams, J.Am. Chem. Soc., 1998, 120, 9605-9613.
Kenzo Hiraoka, “Laser spray: electric field-assisted matrix-assisted laser desorption/ionization”, Journal of Mass Spectrometry, 2004, pp. 341-350, vol. 39.
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