Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-03-10
1993-07-27
Rollins, John W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435 39, 435243, 435 11, 435 12, 436 10, 436 52, 436148, 436149, 436173, 356336, 356335, 356 39, 2504612, C12Q 100, C12Q 106, C12N 100
Patent
active
052310051
ABSTRACT:
A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
REFERENCES:
patent: 4219411 (1980-08-01), Yen
patent: 4452773 (1984-06-01), Molday
patent: 4599307 (1986-07-01), Saunders
patent: 4747685 (1988-05-01), Suzuki
Leif, et al., "Development of Instrumentation . . . "; pp. 1492-1498, Clin Chem. vol. 23, No. 8, 1977.
Coulter Wallace H.
Hajek Constance M.
Hudson James C.
Kortright Kenneth H.
Paul Ronald
Coulter Corporation
Rollins John W.
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