Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-08-12
1993-11-09
Rollins, John W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 30, 435 34, 435 39, 436 10, 436 52, 436148, 436149, 356 39, 356335, 356336, 377 11, 377 12, 2504612, C12Q 100, C12Q 124, C12Q 104, C12Q 106
Patent
active
052601927
ABSTRACT:
A method and apparatus for automatically and rapidly, retrieving counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. A multipart blood cell analysis is obtained with a single sensing parameter by depleting at least one WBC subset population. The percentage of a desired WBC population subset or the overlapping of WBC subset populations also can be obtained by subtracting one or more obscuring WBC subset populations.
The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
REFERENCES:
patent: 4219411 (1980-08-01), Yen
patent: 4452773 (1984-06-01), Molday
patent: 4747685 (1988-05-01), Suzuki
Leif et al, "Clinical Chemistry" 23(8): 1492-1498, 1977.
Coulter Wallace H.
Hajek Constance M.
Rodriguez Carlos M.
Russell Thomas
Coulter Corporation
Rollins John W.
Winburn John T.
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