Method and apparatus for reducing pathogens in a biological...

Details Method and apparatus for reducing pathogens in a biological... Method and apparatus for reducing pathogens in a biological...

2007-09-25

2007-09-25

Tate, Christopher R. (Department: 1655)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S268000, C435S325000

Reexamination Certificate

active

10478917

ABSTRACT:
The present invention is directed to a method and apparatus for disinfection of biological samples, including semen and the like. In a first aspect of the present invention, a method for reducing pathogens in a biological sample includes passing the biological sample through a gradient including an enzyme suitable for removal of at least one pathogen from the sample. In an additional aspect of the present invention, an apparatus for removing pathogens from a biological sample includes a container including a gradient having at least one layer that is suitable for having the biological sample pass through the layer, the at least one layer including an enzyme suitable for removing at least one pathogen from the biological sample.

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“Identification and Distribution of Pz-peptidases A and B in Human Semen” by Bruce A Lessley, et al., Journal of Andrology, Nov./Dec. 1985, vol. 6, pp. 372-378; from the Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma.
“Sperm Recovery Techniques to Maximize Fertilizing Capacity” by David Mortimer, Reprod. Fertil. Dev., 1994, 6, pp. 25-31.
“Identification and Subcellular Localization of the Enzymes Effecting Penetration of the Zona Pellucida by Rabbit Spermatozoa” by Richard Stambaugh, et al, F. Reprod. Fert. 1969 19, pp. 423-432; Division of Reproductive Biology, Department of Obstetrics and Gynecology, University of Pennyslvania School of Medicine, Philadelphia, PA 19104.

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