Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
2006-04-11
2006-04-11
Nguyen, Nam (Department: 1753)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C204S613000
Reexamination Certificate
active
07025864
ABSTRACT:
A preparative electrophoresis apparatus suitable for recovery of molecules from gels comprises two spaced apart electrode compartments connected by a conduit that descends from the bottom of the electrode compartments in a V-shaped or U-shaped form. The conduit serves as a reservoir for collecting electroeluted molecules. Two electrophoresis buffers are used, a first one of a low concentration and density, and a second one of a high concentration and density. The electrode compartments are filled with the first electrophoresis buffer solution while the conduit is filled with the second electrophoresis buffer solution. Under the influence of an electric field, the molecules exit the gel and concentrate in the high concentration buffer. After a certain time, usually a time that is sufficient for driving substantially all desired molecules out of the gel, the electric field is switched off. The high concentration buffer containing the electroeluted molecules is withdrawn from the conduit. The target molecules may be recovered from the high concentration buffer solution.
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Elchrom Scientific A.G.
Fick Anthony
Gilman Michael G.
Nguyen Nam
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