Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1992-09-30
1994-10-11
McGraw, Vincent P.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
2504612, 356417, G01J 330, G01N 2164
Patent
active
053552156
ABSTRACT:
Measurement of the fluorescence of a layer of cells, disposed in a well, with supernatant liquid thereabove, is greatly enhanced in sensitivity by illuminating the cell layer with a beam of light incident thereupon at a first angle and detecting fluorescence emitted by the cells with a detector which views the illuminated cells at a second angle, wherein at least one of the first or second angles is oblique to the cell layer. By so controlling the geometry of the system, the contribution to background fluorescence by the supernatant liquid is greatly minimized. Sensitivity of the technique is further enhanced by restricting the portion of the illuminated cell layer which is viewed by the detector. The technique may be applied to an apparatus for rapidly scanning the fluorescence of a plurality of samples in a multiple well plate.
REFERENCES:
patent: 3604927 (1971-09-01), Hirschfeld
patent: 4343782 (1982-08-01), Shapiro
patent: 4573796 (1986-03-01), Martin et al.
patent: 4626684 (1986-12-01), Landa
patent: 4626693 (1986-12-01), Hirschfeld
patent: 4713781 (1987-12-01), Brizgis et al.
patent: 4758727 (1988-07-01), Tomei et al.
patent: 4786813 (1988-11-01), Svanberg et al.
patent: 4835103 (1989-05-01), Cercek et al.
patent: 4877965 (1989-10-01), Dandliker et al.
patent: 5051162 (1991-09-01), Kambara et al.
patent: 5082628 (1992-01-01), Andreotti et al.
patent: 5091652 (1992-02-01), Mathies et al.
patent: 5097135 (1992-03-01), Makino et al.
patent: 5115137 (1992-05-01), Andersson-Engels
patent: 5190632 (1993-03-01), Fujimiya et al.
NTIS Alert, Jan. 28, 1992; 04,003-290.
Neagle Brad
Schroeder Kirk
Environmental research Institute of Michigan
Hantis K. P.
McGraw Vincent P.
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