Method and apparatus for preparing and culturing cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...

Reexamination Certificate

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Details

C435S399000, C435S402000, C435S286500, C435S299100, C435S299200, C435S818000

Reexamination Certificate

active

07015038

ABSTRACT:
A method and an apparatus for preparing and culturing cells, particularly bone marrow stromal cells. The method includes the steps of placing an oxygenator and a scaffold in a container, such as a syringe, withdrawing bone marrow stromal cells with the syringe, evenly distributing the cells on the scaffold, connecting the syringe with a reservoir with a medium to enrich the syringe with the medium, and promoting the medium level movement in the syringe to culture bone marrow stromal cells.

REFERENCES:
patent: 4065360 (1977-12-01), Kreb, III
patent: 6323022 (2001-11-01), Chang et al.
patent: 2003/0143727 (2003-07-01), Chang
patent: 3607582 (1987-09-01), None
patent: 2000262269 (2000-09-01), None
patent: 734281 (1980-05-01), None
patent: 1131899 (1984-12-01), None
patent: WO 9923243 (1999-05-01), None

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