Method and apparatus for plating and counting aerobic bacteria

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C12K 110

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039620409

ABSTRACT:
A method for determining the concentration of an unknown bacterial solution using only a single bacterial growth plate for the determination. The method comprises depositing a varying amount of bacterial solution on the surface of a solidified agar plate by continuously varying the amount of solution deposited in the shape of a spiral on the agar which is in a rotating Petri dish. Thus, a higher concentration of bacteria per unit length occurs at the center of the spiral and a decreasing concentration per unit length at the edge of the plate. The plate is then incubated and the colonies in a predetermined area of the plate are counted in order to determine the concentration of the unknown solution. The bacterial colonies in a predetermined area may be counted by interrupting a light or laser beam incident to a photodiode, the light being interrupted by the presence of a bacterial colony. The concentration of the unknown solution may then be determined by relating the number of colonies counted to the area on which they were deposited.

REFERENCES:
gall et al., Developments in Industrial Microbiology; pp. 460-469 vol. 11; 1970.

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