Radiant energy – Luminophor irradiation
Patent
1994-08-11
1995-12-26
Fields, Carolyn E.
Radiant energy
Luminophor irradiation
2504591, 356376, 359368, G01N 2164, G02B 2100
Patent
active
054790247
ABSTRACT:
A near-field optical microscope and method of microscopy in which a probe including a flexible cantilever having a sharp tip is positioned in proximity to a sample. In one embodiment, a region of the sample is irradiated with light, and one or more portions of this region are caused to fluoresce. A quenching element is provided at the tip of the probe to quench the fluorescence of these portions within the region. The amount of quenching is determined while the sample is scanned to produce a high resolution image of the irradiated region of the sample. In another embodiment, the fluorescence imparted to one or more portions of the irradiated region is enhanced by the interaction of an optically active element disposed at the tip portion of the cantilever probe which provides for sharper images with greater signal-to-noise ratios. The near-field optical microscopes according to the present invention can also be used to measure the reflection/transmission or absorption characteristics from a sample region within a distance of one wavelength of light away from the sample surface. The microscopes also include means for producing a relative scanning motion between the sample and the probe such as by raster scanner or circular scanning, for example.
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Hansma Paul K.
Hillner Paul E.
Radmacher Manfred
Fields Carolyn E.
The Regents of the University of California
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