Method and apparatus for performing multiple processing...

Details Method and apparatus for performing multiple processing... Method and apparatus for performing multiple processing...

2005-11-15

2005-11-15

Weber, Jon (Department: 1651)

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

C435S041000, C435S069100, C424S068000, C424S418000

Reexamination Certificate

active

06964867

ABSTRACT:
A method is provided for determining crystallization conditions for a protein, the method comprising: performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL; purifying the expressed protein from the separate fermentations; and determining crystallization conditions for the purified protein by submicroliter crystallization experiments.

REFERENCES:
patent: 4490436 (1984-12-01), Kawakami et al.
patent: 5871248 (1999-02-01), Okogbaa et al.
patent: 5945798 (1999-08-01), Stagnitto et al.
patent: 6197517 (2001-03-01), Roberts
patent: 6296673 (2001-10-01), Santarsiero et al.
patent: WO 97/15588 (1997-05-01), None
patent: WO 02/062484 (2002-08-01), None
patent: WO 02/063027 (2002-08-01), None
patent: WO 02/068157 (2002-09-01), None
Cole Parmer Catalog 1999/2000, pp. 228-229, 406, 444-445.

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