Method and apparatus for performing 3-dimensional antibiotic sus

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 32, 4352871, 4352873, 4352883, 53113, 53474, C12Q 124, C12Q 118, C12M 126

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active

054665833

ABSTRACT:
An apparatus for performing 3-dimensional antibiotic susceptibility tests includes a plate with culture medium thereon, the plate rotatably mounted to a support for selective rotation. A knife is located to cut a slit in the culture medium as the plate is rotated, with a dispensing stylus located to dispense inoculum into the slit formed by the knife. A wedge apparatus is mounted adjacent the plate with a wedge-shaped leg depending into the culture medium so as to compress the culture medium towards the center of the plate, to thereby force the walls of the slit together after the inoculum has been deposited within the slit. A method for performing modified 3-dimensional antibiotic susceptibility tests includes the step of uniformly spreading a test microorganism over the top surface of culture medium on a plate. A slit is then formed in the culture medium and a uniform stream of suspending medium with a test microorganism therein is dispensed in and along the length of the slit. At least one antibiotic disk is then placed on the culture medium adjacent the slit, and the medium is incubated as desired.

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Product brochure: Spiral System Instruments 1990.
Product brochure: "Reduce the Cost of Microbial Assays" Spiral System Instruments, Inc.
Journal of Antimicrobial Chemotherapy--1984, 13, 45-54 "3-Dimensional Susceptibility T4sting of B-Lactam Antibiotics".

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