Method and apparatus for patch-clamp measurements on cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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Details

C435S287100, C435S288500, C436S063000

Reexamination Certificate

active

07611861

ABSTRACT:
The invention shows a method for patch-clamp experiments on cells or similar structures, where at least one cell is inserted into the lumen of a capillary and is positioned inside the capillary so, that a sufficiently tight seal with a resistance exceeding 1, preferably 10 GigaOhm develops between cell membrane and inner surface of the capillary. Along its length said capillary has at least at one position a smaller inner diameter than the outer diameter of said cell. Preferably the cell is inserted and positioned in the capillary by pressure, suction, sedimentation or centrifugation of a suspension or solution containing said cell. An apparatus for performing experiments using this method is described.

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