Method and apparatus for particle measurement employing...

Image analysis – Applications – Biomedical applications

Reexamination Certificate

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C356S335000

Reexamination Certificate

active

07379577

ABSTRACT:
A system and method for measuring small particles suspended in a fluid are disclosed. The system employs optical imaging using diffraction enlargement. A sample of small particles illuminated by a light source is imaged onto a pixel array of detector elements using an imaging optical system having a reduced magnification not sufficient for forming a large enough image of a smallest particle of interest. A low-aperture imaging optics with NA<0.05 is used to add diffraction enlargement to the image corresponding to at least 5 pixels to enable accurate measurement of images of smallest particles of interest, and to increase an optical sampling volume. Suitably programmed processor is used for determining at least a pixel count for each of the diffraction-enlarged images, and for generating a number, size or distribution of particles accounting for pre-determined diffraction enlargement of particle images of different sizes. The method enables analysis of large samples of small particles in one measurement.

REFERENCES:
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patent: 6061130 (2000-05-01), Plate et al.
patent: 7064826 (2006-06-01), Rabinski et al.
patent: 2002/0159047 (2002-10-01), Dubois
patent: 2004/0008867 (2004-01-01), Fein et al.

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