Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-11-23
1994-08-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 723, 435 724, G01N 3350, G01N 3380, G01N 128, G01N 2100
Patent
active
053407199
ABSTRACT:
An optical screening method and apparatus for identifying both the morphology and selected characteristics or properties expressed by cells. The cells are combined with one or more different sets of microspheres, each set having a reactant bond thereto which will bind to a specific molecule which can exist on one or more types of the cells. The cells and microspheres are formed as a smear on a slide, stained with a histological type stain and optically viewed to identify the type of cells to which the different sets of microspheres do or do not bind. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof. A fast cell screening method is provided by the observation of clumping of the cells and microspheres, where present. Also, cells can be screened by utilizing a first reactant which will bind to a specific cell molecule and binding a second reactant to the first reactant, which second reactant is bound to a set of microspheres.
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Hajek Constance M.
Russell Thomas
Coulter Corporation
Moore William W.
Wax Robert A.
Winburn John T.
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