Method and apparatus for microphotometering microscope specimens

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356308, 356318, 358107, H04N 718

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RE0342149

ABSTRACT:
A method of microphotometering individual volume elements of a microscope specimen 10, comprising generating a luminous dot or cursor and progressively illuminating a plurality of part elements in the focal plane 11 of the microscope through the specimen. The mutual position between the specimen and the focal plane is then changed and a plurality of part elements in the focal plane are illuminated. Reflected and/or fluorescent light and transmitted light respectively created by the illumination is collected, detected and stored for generating a three-dimensional image of that part of the specimen composed of the volume elements. Illumination of multiples of part elements is implemented by deflecting the cursor and/or by moving the specimen. The change in the relative mutual position between the specimen and the focal plane of the microscope is effected either by displacing the specimen or the objective. Apparatus for carrying out the method include a specimen table 301, a microscope objective and light source 31-32-33. The table or the objective are arranged for stepwise movement along the main axis of the microscope synchronously with punctilinear light scanning of the specimen. The table is arranged for stepwise movement at right angles to the main axis and/or the light source is arranged for deflection over the focal plane 21 through the specimen.

REFERENCES:
patent: 2969708 (1961-01-01), Polanyi et al.
patent: 3013467 (1961-12-01), Minsky
patent: 3049047 (1962-08-01), Polanyi et al.
patent: 3719776 (1973-03-01), Fujiyasu et al.
patent: 3764512 (1973-10-01), Greenwood et al.
patent: 3782823 (1974-01-01), Kantorski et al.
patent: 3790281 (1974-02-01), Kessler et al.
patent: 3926500 (1975-12-01), Frosch et al.
patent: 3947628 (1976-03-01), Alien et al.
patent: 3980818 (1976-09-01), Browning
patent: 4045772 (1977-08-01), Bouton et al.
patent: 4068381 (1978-01-01), Ballard et al.
patent: 4125828 (1978-11-01), Resnick et al.
patent: 4141032 (1979-02-01), Haeusler
patent: 4160263 (1979-07-01), Christy et al.
patent: 4194217 (1980-03-01), van den Bosch
patent: 4207554 (1980-06-01), Resnick et al.
patent: 4211924 (1980-07-01), Muller et al.
patent: 4218112 (1980-08-01), Ruker
patent: 4223354 (1980-09-01), Noble
patent: 4236179 (1980-11-01), Dreyfus et al.
patent: 4255971 (1981-03-01), Rosencwaig
patent: 4284897 (1981-08-01), Sawamura et al.
patent: 4311358 (1982-01-01), Gibbons et al.
patent: 4314763 (1982-02-01), Steigmeier et al.
patent: 4348263 (1982-09-01), Draper et al.
patent: 4350892 (1982-09-01), Kay et al.
patent: 4354114 (1982-10-01), Karnaukhov et al.
patent: 4362943 (1982-12-01), Presby
patent: 4366380 (1982-12-01), Mirkin
patent: 4379135 (1983-04-01), Sasaki et al.
patent: 4379231 (1983-04-01), Shii et al.
patent: 4381963 (1983-05-01), Goldstein et al.
patent: 4405237 (1983-09-01), Manuccia et al.
patent: 4406015 (1983-09-01), Koga
patent: 4406525 (1983-09-01), Itoh et al.
patent: 4407008 (1983-09-01), Schmidt
patent: 4485409 (1984-11-01), Schumacher
"Electronic Image Processing of Scanning Optical Microscope Images", I. J. Cox, presented at International Conference on Electronic Image Processing, Jul. 26-28, 1982.
D. K. Hamilton et al., "Three-Dimensional Surface Measurement Using the Confocal Scanning Microscope", Applied Physics B27, pp. 211-213 (1982).
G. J. Brakenhoff, "Imaging modes in confocal scanning light microscopy (CSLM)", Journal of Microscopy, vol. 117, pt. 2, Nov. 1979, pp. 233-242.
"Tandem Scanning Reflected Light Microscopy of Internal Features in Whole Bone and Tooth Samples" A. Boyde, M. Petran, M. Hadravsky, Journal of Microscopy, vol. 132, part 1, Oct., 1983, pp. 1-7.
"Laser Scanning Microscopy", W. Jerry Alford, Richard D. Vanderneut, Vincent J. Zaleckas, Proceedings of the IEEE, vol. 70, No. 6, Jun., 1982, pp. 641-651.
"Digital Image Processing of Confocal Images", I. J. Cox, C. J. R. Sheppard, Image and Vision Computing, 1983, Butterworth & Co., Ltd. (Publishers), pp. 52-56.
"Experimental Observations of the Depth-Discrimination Properties of Scanning Microscopes", D. K. Hamilton, T. Wilson, C. J. R. Sheppard, Optics Letters, Dec., 1981, vol. 6, No. 12, pp. 625-626.
"A Laser Flying Spot Scanner for Use in Automated Fluorescence Antibody Instrumentation", A. F. Slomba, D. E. Wasserman, G. I. Kaufman, J. F. Nester, Journal of the Association for the Advancement of Medical Instrumentation, vol. 6, No. 3, May-Jun., 1972, pp. 230-234.
"Depth of Field in the Scanning Microscope", C. J. R. Sheppard, T. Wilson, Optics Letters, vol. 3, No. 3, Sep., 1978, pp. 115-117.
"Scanning Laser Microscope for Biological Investigations", P. Davidovits, M. D. Egger, Applied Optics, vol. 10, No. 7, Jul., 1971, pp. 1615-1619.
"Tandem-scanning Reflected-light Microscope", Mojmir Petran, Milan Hadravsky, M. David Egger, Robert Galambos, Journal of the Optical Society of America, vol. 58, No. 5, May, 1968, pp. 661-664.
"Scanning Laser Microscope", Paul Davidovits, M. David Egger, Nature, vol. 223, Aug. 1969, p. 831.
"Image-analyzing Microscopes", Phillip G. Stein, Analytical Chemistry, vol. 42, No. 13, Nov., 1970, pp. 103A-106A.
"PHOIBOS, A Microscope Scanner Designed for Micro-fluorometric Applications, Using Laser Induced Fluoroscence", N. Aslund, K. Carlsson, A. Liljeborg, L. Majlof, Physics IV, Royal Institute of Technology, S-100 44 Stockholm 70, 1983, pp. 338-343.
"Scanning Optical Microscope Incorporating a Digital Framestore and Microcomputer", I. J. Cox, C. J. R. Sheppard, 2219 Applied Optics vol. 22, No. 10, May, 1983, New York, U.S.A., pp. 1474-1478.
"Optical Microscopy with Extended Depth of Field", C. J. R. Sheppard, D. K. Hamilton, I. J. Cox, Proc. R. Soc. Lond. A, vol. 387, pp. 171-186.
"Three-dimensional Architecture of a Polytene Nucleus", David A. Agard, John W. Sedat, Nature, vol. 302, Apr. 21, 1983, pp. 676-681.
"Dynamic Focusing in the Confocal Scanning Microscope", T. Wilson D. K. Hamilton, Journal of Microscopy, vol. 128, pt. 2, Nov., 1982, pp. 139-143.
"Morphoquant-An Automatic Microimage Analyzer of VEB Carl Zeiss Jena", Shura Agadshanyan, Peter Dopel, Peter Gretscher, Werner Witsack, JR 6, 1977, pp. 270-276.
"Automatic Moving Part Measuring Equipment", H. M. Nier, IBM Technical Disclosure Bulletin, vol. 22, No. 7, Dec., 1979, pp. 2856-2857.
The Engineering Index Annual, 1983, pp. 3434 and 4491.
Digital Image Processing, Kenneth R. Castleman, 1979 Prentice-Hall, Inc.

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