Method and apparatus for microphotometering microscope specimens

Details Method and apparatus for microphotometering microscope specimens Method and apparatus for microphotometering microscope specimens

1985-02-21

1986-12-23

Britton, Howard W.

Facsimile and static presentation processing

Static presentation processing

Attribute control

356308, 356318, 358107, H04N 718

Patent

active

046315810

ABSTRACT:
A method for microphotometering individual volume elements of a microscope specimen 10, comprising generating a luminous dot or cursor and progressively illuminating a plurality of part elements in the focal plane 11 of the microscope through the specimen. The mutual position between the specimen and the focal plane is then changed and a plurality of part elements in the focal plane are illuminated. Reflected and/or fluorescent light and transmitted light respectively created by the illumination is collected, detected and stored for generating a three-dimensional image of that part of the specimen composed of the volume elements. Illumination of multiples of part elements is implemented by deflecting the cursor and/or by moving the specimen. The change in the relative mutual position between the specimen and the focal plane of the microscope is effected either by displacing the specimen or the objective. Apparatus for carrying out the method include a specimen table 301, a microscope objective and light source 31-32-33. The table or the objective are arranged for stepwise movement along the main axis of the microscope synchronously with punctilinear light scanning of the specimen. The table is arranged for stepwise movement at right angles to the main axis and/or the light source is arranged for deflection over the focal plane 21 through the specimen.

REFERENCES:
patent: 4141032 (1979-02-01), Haeusler
patent: 4194217 (1980-03-01), van den Bosch
patent: 4218112 (1980-08-01), Ruker
patent: 4223354 (1980-09-01), Noble
patent: 4407008 (1983-09-01), Schmidt

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