Method and apparatus for measuring the electrophoretic mobility

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2726

Patent

active

046612254

ABSTRACT:
An apparatus for measuring the electrophoretic mobility of migrating particles in a suspending medium under the influence of an applied electric field comprising an electrophoresis chamber, means for illuminating the electrophoresis chamber, a microscope having an objective lens system and an ocular lens system, said microscope being positioned to view the electrophoresis chamber along an optical path extending from the electrophoresis chamber through the objective lens system and toward the ocular lens system, means for generating moving bands of visible energy into the ocular lens system of the microscope, means for adjusting the speed of the moving bands of visible energy; and means for determining the speed of the moving bands.
A method for measuring the electrophoretic mobility of migrating particles in a suspending medium under the influence of an applied electric field which comprises introducing a suspension medium containing colloidal particles into an electrophoresis chamber, positioning the electrophoresis chamber on a spring-loaded plate mechanism, illuminating the contents of the electrophoresis chamber, viewing the illuminated colloidal particles in the electrophoresis chamber through a microscope, applying a voltage potential across the electrodes of the electrophoresis chamber, to cause migration of the colloidal particles, generating moving bands of visible energy in the ocular lens system of the microscope, adjusting the speed of the moving bands until they match the speed of the migrating particles, and determining the speed of the moving bands and thus the corresponding mobility of the colloidal particles.

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