Optics: measuring and testing – By dispersed light spectroscopy – Utilizing a spectrometer
Patent
1991-03-19
1992-03-17
Evans, F. L.
Optics: measuring and testing
By dispersed light spectroscopy
Utilizing a spectrometer
356328, 356407, 356419, G01N 2127
Patent
active
050962949
ABSTRACT:
A non-invasive method and apparatus are described for measuring the oxygen concentration in the infected cells of the nodules of nitrogen fixing plants in the laboratory or field. In many cases, this information can be used to estimate the nitrogenase activity, and therefore the nitrogen fixation rate, in these nodules since recent studies have shown that the oxygen concentration limits and controls nitrogenase activity under most environmental conditions. Using the same apparatus, a method to measure nodule respiration and nodule oxygen permeability is also described. The nodule oxygen concentration in the infected cells of nodules is maintained at very low levels and has not been measurable previously by non-invasive techniques. The fractional oxygenation of plant hemoglobin is measured spectroscopically using one or more modulated light sources, a number of optical fibres to convey the light to and from the nodules, a photodetector to measure the light passing through the nodule and a detection system such as a photodiode coupled to a microcomputer or a lock-in amplifier to process the output signal.
REFERENCES:
patent: 4938699 (1990-07-01), Cassagne
Denison R. Ford
Hunt Stephen
Layzell David B.
Palmer Gerry
Evans F. L.
Hicks Richard J.
Queen's University at Kingston
The United States of America as represented by the Department of
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