Method and apparatus for measuring nitric oxide production...

Details Method and apparatus for measuring nitric oxide production... Method and apparatus for measuring nitric oxide production...

2007-07-24

2007-07-24

Beisner, William H. (Department: 1744)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S286500, C435S293100, C435S807000, C422S079000, C422S088000

Reexamination Certificate

active

10996395

ABSTRACT:
An apparatus and method for measuring nitric oxide production and oxygen consumption in cultures of adherent cells continuously and without destroying the cells. The method involves flowing growth media through a tube having adherent cells are adhered to the inner surface thereof and then contacting the growth media with an NO or O2sensor to detect the concentration of NO or O2in the growth media.

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Hallman, T.M. et al: “Oxygen dependence of nitric oxide synthesis in raw 264.7 cells.” FASEB Journal, vol. 13, No. 4, Part 1, Mar. 12, 1999.

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