Method and apparatus for measuring fluorescence polarization...

Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence

Reexamination Certificate

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C356S364000

Reexamination Certificate

active

07427509

ABSTRACT:
Disclosed relates to a method and an apparatus for measuring fluorescence polarization FP in a lab-on-a-chip and, more concretely, to a method and an apparatus that measure quantitatively interactions between biomolecules and fluorescently labeled biomaterials and enzyme activities using the measurement of fluorescence polarization FP. The method and the apparatus of the invention provide rapid assays with minute amounts of samples using an automated device compared to conventional methods. Accordingly, the method and the apparatus of the invention can be usefully applied to the measurement of interactions between biomolecules and to the protease assays using a protein substrate.

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Wan and Le, “Studies of Protein-DNA Interactions by Capillary Electrophoresis/Laser-Induced Fluorescence Polarization,” Analytical Chemistry, 72(22): 5583-5589 (2000).
Yadavalli and Pishko, “Biosensing in Microfluidic Channels Using Fluorescence Polarization,” Analytica Chimica Acta, 507: 123-128 (2004).

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