Radiant energy – Luminophor irradiation
Patent
1998-10-14
1999-11-23
Evans, F. L.
Radiant energy
Luminophor irradiation
2504591, 356318, G01N 2164
Patent
active
059904844
ABSTRACT:
The present invention relates to a method and apparatus for efficiently measuring the lifetime of the fluorescence emitted from a fluorescent material in response to pulsed light. The measuring apparatus according to the present invention includes, at least, an excitation light source whose excitation power is regulated such that at least one fluorescence photon can be detected per light pulse on average. While repeatedly irradiating a sample containing the fluorescent material with excitation pulses of light from the excitation light source, a fluorescence detection time from irradiation to fluorescence detection and the number of detected fluorescence photons are measured for each excitation pulse of light. Then, on the basis of a data group composed of pairs of the measurement data of fluorescence photon detection time and fluorescence photon number, both of which are measured for each occurrence of excitation pulse of light, the lifetime and fluorescence decay curve of the fluorescence emitted from the fluorescent material are determined.
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Davis et al., "Correction Methods for Photon Pile-Up in Lifetime Determination by Single-Photon Counting", Oct. 1969, pp. 101-109.
Coates, "The Correction for Photon `Pile-Up` In the Measurement of Radiative Lifetimes", Journal of Scientific Instruments (Journal of Physics E) 1968 Series 2, vol. 1, pp. 878-879.
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Birch et al., "Time-Domain Fluorescence Spectroscopy Using Time-Correlated Single-Photon Counting", Topics in Fluorescence Spectroscopy, vol. 1: Techniques, 1991, pp. 1-95.
Evans F. L.
Laboratory of Molecular Biophotonics
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