Method and apparatus for measuring binding affinity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 4, 435 71, 435970, 435973, 435975, 436164, 436518, 436527, 436531, 436808, 436809, 436810, 436823, 422 52, 422 8206, 422 99, 530333, 530334, G01N 33543, G01N 33566

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053246330

ABSTRACT:
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.

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