Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1991-11-22
1994-06-28
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 71, 435970, 435973, 435975, 436164, 436518, 436527, 436531, 436808, 436809, 436810, 436823, 422 52, 422 8206, 422 99, 530333, 530334, G01N 33543, G01N 33566
Patent
active
053246330
ABSTRACT:
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.
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Fodor Stephen P. A.
Mazzola Laura T.
Affymax Technologies N.V.
Kaster Kevin
Kepplinger Esther L.
Liebeschuetz Joe
Norviel Vern
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