Method and apparatus for measuring binding affinity

Details Method and apparatus for measuring binding affinity Method and apparatus for measuring binding affinity

1991-11-22

1994-06-28

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 71, 435970, 435973, 435975, 436164, 436518, 436527, 436531, 436808, 436809, 436810, 436823, 422 52, 422 8206, 422 99, 530333, 530334, G01N 33543, G01N 33566

Patent

active

053246330

ABSTRACT:
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.

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