Liquid purification or separation – With means to add treating material – Chromatography
Patent
1996-10-09
1998-03-24
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
210656, B01D 1508
Patent
active
057308675
ABSTRACT:
A method and apparatus for low pressure liquid chromatography which is particularly suitable for the testing of body fluids and in particular blood, the method comprising the steps of passing a sample through a chromatography column containing a packing material so that a constituent of the sample is retained by the packing material and passing a buffer solution having a varying concentration through the column. When the concentration of the buffer solution reaches a particular level, the constituent will be disassociated from the packing material and flows out of the column through a detector. The detector measures the light absorption of the fluid and by relating the time varying light absorption of the fluid to the time varying concentration of the buffer solution, it is possible to identify the constituent. Samples of body fluid are conveniently taken by puncturing the skin and applying a capillary tube to the fluid which emerges. The capillary tube is then placed in a pre-filled sample vial containing a solution for processing the body fluid and, after a certain time, the vial is placed in the chromatography apparatus and is drawn into the apparatus for testing. The apparatus is controlled by a microprocessor operating according to a stored program, the chromatography column used is a two part plastic column about 5 mm long and adapted to operate at a pressure of about 15 psi. The two part nature of the column allows it to be filled particularly easily.
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Drew Keith Raymond
Hjelm Nils Magnus
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