Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1983-11-10
1986-12-02
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436535, 436537, 436807, 436804, 436 56, 436 57, 436172, 436178, 436531, 422 58, 422 68, G01N 33543, G01N 33544, G01N 33542
Patent
active
046265139
ABSTRACT:
A process and apparatus has been developed for radioassay of ligand in solution which eliminates the separation step required in conventional techniques. A chamber is provided containing a quenching solution, a plurality of ligand molecules and a plurality of receptor molecules. One of pluralities forms a free species labelled with a beta particle emitter while the other is immobilized on a solid support, e.g., the chamber wall or a microbead, within the chamber. Ligand introduced with the sample competes with ligand molecules already in the chamber for receptor sites on the receptor molecules and the free species is allowed to diffuse about the chamber. A beta particle detector in communication with the chamber at a fixed position detects only those beta particles emitted from within the quenching distance of the quenching solution. The quenching properties of the solution are used in place of the conventional separation step. The process and apparatus are easily adapted for continuous monitoring of ligand level and are particularly well suited for use in radioimmunoassays. The apparatus can be miniturized allowing implantation in an animal body and in vivo monitoring of ligand level in bodily fluids.
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Burton James A.
Hoop, Jr. Bernard
Massachusetts General Hospital
Nucker Christine M.
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