Method and apparatus for isocratic affinity chromatography

Details Method and apparatus for isocratic affinity chromatography Method and apparatus for isocratic affinity chromatography

1988-02-26

1989-11-07

Richman, Barry S.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436161, 422 70, 210635, 2101982, 2105021, 502403, G01N 3353, B01D 1508

Patent

active

048792470

ABSTRACT:
The invention relates to a high performance affinity chromatographic system characterized by an immobilized active substance interacting weakly (K.sub.diss >10.sup.-3 M) with the desired structure of the type or types of molecule to be separated. The substance is covalently bound in its active state on a carrier of a solid, preferably porous, organic or inorganic material thereby making it suitable for use in the so-called HPLC or HPLAC technique for the separation of e.g. biomolecules. Examples of useful inorganic materials are silica or glass, or organic materials such as plastics or polysaccharides. Separation takes place by the mobile liquid being led through the column packed with the immobilized active substance of weak affinity for the complementary substances. Examples of such immobilized ligands are antibodies or fragments thereof, antigens or fragments thereof and various receptor substances.

REFERENCES:
Turley et al., "Biochemistry", 1987, 26, 2997-3005.
Rosemeyer et al., "Annal Biochem", 1981, 115(2), pp. 339-346.
Sariban-Sohraby et al., "Biochemistry", 8/12/86, 25(16), pp. 4639-46.
Kruckeberg et al., "Biochemistry", 1978, 17(21), pp. 4376-82.
"Affinity Chromatography", Rodney R. Walters, Analytical Chemistry, vol. 57, No. 11, Sep. 1985, pp. 1099A-1114A.
"Fast Affinity Chromatography", Biotechnology, vol. 4, pp. 519-521.

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