Method and apparatus for in vitro fertilization

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

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4351722, 4353052, 435374, 435373, 600 33, 600 34, 600 35, 604403, 604404, C12N 500, C12N 1500, A61B 1900

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active

056270666

ABSTRACT:
This invention relates to a method and apparatus for concentrating motile erm from a sperm sample, including concentrating sperm in the vicinity of one or more oocytes for in vitro fertilization. The method comprises placing a volume of fertilization medium in a container, said container having at least one microchamber and being shaped such that the fertilization medium, when placed in the container, fills the microchamber and a portion of the container outside of the microchamber, such that when a sperm sample is placed in the medium outside the chamber motile sperm swim into the microchamber, where they may be harvested or, if an oocyte is in the microchamber, in vitro fertilization occurs.

REFERENCES:
Laufer et al., In-Vitro Fertiliz., 481-505, 1990.
Banister, J. Exp. Zool, (1979) 210:259-264.

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