Method and apparatus for improving electrophoresis resolution

Details Method and apparatus for improving electrophoresis resolution Method and apparatus for improving electrophoresis resolution

1998-06-11

2000-02-15

Warden, Jill

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204616, G01N 2726

Patent

active

060248548

ABSTRACT:
Electrophoretic separation of an analyte species in a sample is achieved with increased resolution by loading the sample onto a loading site of an electrophoresis gel, said loading site having a gel/buffer interface and then applying a focusing electric field to a first pair of electrodes to cause the analyte species to migrate to a narrow region disposed at or near the loading site to produce a focused sample. Then a separation electric field is applied to cause the analyte species in the focused sample to migrate through the electrophoresis gel and to be separated into bands. This method is preferably performed in an electrophoresis apparatus that is particularly adapted to practicing the method by virtue of the a pair of focusing electrodes which are positioned to cause migration of sample to a narrow region near the buffer/gel interface within the sample loading site of the gel. This actual location of this narrow region may be in a buffer region over the gel, or just within the gel near the loading site, for example within 500 microns of the loading site.

REFERENCES:
patent: 4908112 (1990-03-01), Pace
patent: 5104512 (1992-04-01), Gombocz et al.
patent: 5217591 (1993-06-01), Gombocz et al.
patent: 5328578 (1994-07-01), Gordon
patent: 5549796 (1996-08-01), Chu et al.
patent: 5833826 (1998-11-01), Nordman

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