Method and apparatus for identifying chromosomes or cells

Radiant energy – Luminophor irradiation – With ultraviolet source

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2504591, G01N 2164

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active

050417339

ABSTRACT:
A method and an apparatus for sorting chromosomes or cells by utilizing a difference in fluorescent profile-patterns. A linear laser beam having a high beam intensity is formed from several fringe patterns, and fluoresence intensities from local portions of chromosome or cell are measured in real-time. Resolution of chromosome or cell is improved. A type of flowing chromosome or cell which permits the rotational conditions within a range can be sorted accurately and rapidly.

REFERENCES:
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patent: 4596036 (1986-06-01), Norgren et al.
patent: 4778593 (1988-10-01), Yamashita et al.
Dean, P. N. et al., "High Resolution Dual Laser Flow Cytometry", J. Histochem. Cytochem., vol. 26, pp. 622-627 (1978).
Lebo, R. V. et al., "High-Resolution Chromosome Sorting and DNA Spot-Blot Analysis Assign McArdle's Syndrome to Chromosome II", Science, vol. 225, pp. 57-59 (1984).
Wheeless, L. L., "Slit-Scanning and Pulse Width Analysis", In: Melamed, M. R. et al., Flow Cytometry and Sorting (New York, John Wiley & Sons, 1979) pp. 125-135.
Cambier, J. L., et al., "A Multidimensional Slit-Scan Flow System", J. Histochem. Cytochem., vol. 27, pp. 321-324 (1979).
Norgren, R. M. et al., "Restoration of Profiles from Slit-Scan Flow Cytometry", IEEE Transactions on Biomedical Engineering, vol. BME-29, pp. 101-106 (1982).

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