Method and apparatus for heterogeneous chemiluminescence assay

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436530, 436537, 422 56, 422 58, 422 57, 422 61, G01N 33543

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050894242

ABSTRACT:
Apparatus and method for performing a chemiluminescence assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

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John B. Birks, Photophysics of Aromatic Molecules, Chapter 2, Photophysical Processes, Wiley-Interscience, N.Y. 1970.

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