Method and apparatus for growing biomass particles

Details Method and apparatus for growing biomass particles Method and apparatus for growing biomass particles

1993-08-06

1997-08-05

Hutzel, Paula K.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of culturing cells in suspension

435403, 435261, 4352972, 4353081, C12N 502, C12M 302

Patent

active

056541970

ABSTRACT:
The present invention provides an improved method and apparatus for growing biomass particles, particularly microcarrier-bound cells, in an agitated suspension culture vessel in which fresh culture medium is added and spent culture medium is withdrawn continuously or semi-continuously. The improvement comprises withdrawing the spent culture medium through a particle settling chamber located within the vessel and at least partially immersed in the agitated culture medium therewithin. The particle settling chamber comprises a hollow container with a bottom opening through which biomass particles, such as microcarrier-bound cells, settle by gravity back into the agitated culture medium and a top opening through which particle-free spent culture medium is withdrawn form the vessel. The settling chamber is configured such that the fluid velocity of culture medium entering the settling chamber through the bottom opening is significantly less than the biomass particle settling velocity.

REFERENCES:
patent: 4335215 (1982-06-01), Tolbert et al.
patent: 4814278 (1989-03-01), Hamamoto et al.
Tokashiki et al. "High Density Culture of Mouse-Human Hybridoma Cells Using a New Perfusion Culture Vessel" (Mar. 14, 1988) pp. 337-341.
Tolbert et al., "Manufacture of Pharmacologically Active Proteins by Manmalian Cell Culture" Biopharm Mfg., Sep. 1987.
Feder et al., "The Large-Scale Cultivation of Mammalian Cells", Scientific American. vol. 248, No. 36 (Jan. 1983) pp. 36-43.

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