Method and apparatus for generating electric fields and flow...

Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C204S450000, C422S105000

Reexamination Certificate

active

07597791

ABSTRACT:
A method and apparatus for generating tunable, uniform electric fields in fluidic applications for rapid separation of molecules, such as DNA, is provided. A region receives the molecules to be separated, the molecules being injected into the region by an injection channel connected thereto. Fluidic microchannels or resistor arrays connected to sides of the region inject currents into the region and produce electric fields in the region that can be oriented at any angle. The electric fields can separate the molecules according to size, and can be used to move or manipulate the molecules within the region. Further, the molecules can be separated by controlling fluid flows within the region to manipulate the molecules. One or more reservoirs can be attached to the fluidic microchannels for collecting the molecules after separation, movement, or manipulation.

REFERENCES:
patent: 2555487 (1951-06-01), Haugaard, et al.
patent: 3450624 (1969-06-01), Natelson
patent: 3458427 (1969-07-01), Strickler
patent: 3498905 (1970-03-01), Strickler
patent: 3519549 (1970-07-01), Grassmann, et al.
patent: 3563872 (1971-02-01), Huebner
patent: 3847773 (1974-11-01), Snyder
patent: 4061560 (1977-12-01), Hannig et al.
patent: 4148703 (1979-04-01), Trop et al.
patent: 4315812 (1982-02-01), Karlson
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4523320 (1985-06-01), Stappaerts
patent: 4693804 (1987-09-01), Serwer
patent: 4732656 (1988-03-01), Hurd
patent: 4737251 (1988-04-01), Carle et al.
patent: 4740283 (1988-04-01), Laas et al.
patent: 4830726 (1989-05-01), Stamato et al.
patent: 5011586 (1991-04-01), Finney et al.
patent: 5084157 (1992-01-01), Clark et al.
patent: 5106468 (1992-04-01), Chimenti
patent: 5116471 (1992-05-01), Chien et al.
patent: 5122248 (1992-06-01), Karger et al.
patent: 5165898 (1992-11-01), Chu et al.
patent: 5178737 (1993-01-01), Lai
patent: 5180480 (1993-01-01), Manz
patent: 5405519 (1995-04-01), Schwartz
patent: 5427663 (1995-06-01), Austin et al.
patent: 5671086 (1997-09-01), Parvin et al.
patent: 5837115 (1998-11-01), Austin et al.
patent: 5972190 (1999-10-01), Richman
patent: 6027623 (2000-02-01), Ohkawa
patent: 6110339 (2000-08-01), Yager et al.
patent: 6156273 (2000-12-01), Regnier et al.
patent: 6176990 (2001-01-01), Yager et al.
patent: 6254754 (2001-07-01), Ross et al.
patent: 6280590 (2001-08-01), Cheng et al.
patent: 6328868 (2001-12-01), Weber
patent: 6540896 (2003-04-01), Manz et al.
patent: 6596144 (2003-07-01), Regnier et al.
patent: 6685810 (2004-02-01), Noca et al.
patent: 6881317 (2005-04-01), Huang et al.
patent: 2005/0161331 (2005-07-01), Huang et al.
patent: 0560974 (1997-01-01), None
patent: WO 02/50095 (2002-06-01), None
patent: WO 03/035228 (2003-05-01), None
Manz et al, J. Micromech. Microeng. 4 (1994), pp. 257-265.
Huang, et al., “Generation of Large-Area Tunable Uniform Electric Fields in Microfluidic Arrays for Rapid DNA Separation,” Technical Digest of the International Electron Devices Meeting, pp. 363-366, (2001).
Bakajin, et al., “Microfabricated Arrays for Fractionation of Large DNA Molecules Via Pulsed Field Electrophoresis,” Biological Physics: Third International Symposium, 1999, pp. 243-248.
Duke, et al., “Microfabricated Sieve for the Continuous Sorting of Macromolecules,” Physical Review Letters, vol. 80, No. 7, Feb. 16, 1998, pp. 1552-1555.
Duke, et al., “Pulsed-Field Electrophoresis in Microlithographic Arrays,” Electrophoresis, vol. 17, 1996, pp. 1075-1079.
Viovy, “Electrophoresis of DNA and Other Polyelectrolytes: Physical Mechanisms,” Views of Modern Physics, vol. 72, No. 3, Jul. 2000, pp. 813-872.
PCT International Search Report mailed Dec. 17. 2002, in connection with International Publication No, WO 03/035228 A1.
PCT Written Opinion mailed Sep. 25, 2003, in connection with International Publication No. WO 03/035228 A1.
PCT international Preliminary Examination Report completed Jan. 22, 2004, in connection with International Publication No, WO 03/035228 A1.
PCT International Search Report mailed Apr. 19, 2002, connection with International Publication No. WO 02/50095 A1.
PCt Written Opinion mailed Apr. 17, 2003, in connection with International Publication No, WO 02/50095 A1.
PCT International Preliminary Examination Report completed Jun. 2004, in connection with International Publication No. WO 02/50095 Al.
European Search Report dated May 18, 2005 in connection with European Application No. EP 01 99 1239.
Notice of Allowance dated Dec. 14, 2004 from U.S. Patent No. 6,881,317.
Office Action dated Feb. 23, 2004, from U.S. Patent No. 6,881,317.
Office Action dated Aug. 20, 2003, from U.S. Patent No, 6,881,317.
Office Action dated Mar. 24, 2003, from U.S. Patent No. 6,881,317.
Notice of Allowance dated Nov. 9, 2007 from pending U.S. Appl. No. 11/075,682.
Office Action dated Oct. 10, 2007, from pending U.S. Appl. No. 11/015,682.
Office Action dated Jun. 20, 2007, from pending U.S. Appl. No. 11/075,682.
Advisory Action dated Apr. 19, 2007, from pending U.S. Appl. No. 11/075,682.
Inteview Summary dated Apr. 10, 2007, from pending U.S. Appl. No. 11/075,682.
Office Action dated Dec. 12, 2006, from pending U.S. Appl. No. 11/075,682.
Office Action dated Aug. 25, 2006, from pending U.S. Appl. No. 11/075,682.
Office Action dated Feb. 7, 2006, from pending U.S. Appl. No. 11/075,682.
Notice of Allowance and Fees Due mailed Mar. 20, 2008 from co-pending U.S. Appl. No. 11/075,682.
Notice of Allowability mailed Mar. 20, 2008 from co-pending U.S. Appl. No. 11/075,682.
Office Action dated Jul. 22, 2008, issued by Japanese Patent Office in connection with pending Japanese Patent Application No. 2002-551988 (3 pages).
English translation of Office Action dated Jul. 22, 2008, issued by Japanese Patent Office in connection with pending Japanese Patent Application No. 2002-551988 (3 pages).
Beverley, “Characterization of the ‘unusual’ mobility of large circular DNAs in pulsed field-gradient electrophoresis”, Nucleic Acids Research, 1988, pp. 925-939, vol. 16, No. 3 (15 pages).
Office Action dated Aug. 20, 2008, issued by the European Patent Office in connection with European Patent Application No. 09 991 239. 3-2204 (6 pages).
Beverley, “Characterization of the 'Unusual' Mobility of Large Circular DNAs in Pulsed Field-Gradient Electrophoresis,” Nucleic Acids Research, vol. 16, No. 3, (1988) pp. 925-939 (15 pages).
Cognasso, et al., “L' elettroforesi su carta in corrente alternata asimmetrica,” Progresso Medico, vol. XVII, No. 8, Apr. 30, 1961, pp. 255-258 (5 pages) (including English summary).
Schwartz, et al., “Separation of Yeast Chromosome-Sized DNAs by Pulsed Field Gradient Gel Electrophoresis,” Cell, vol. 37, May 1984. pp. 67-75 (9 pages).
Van der Ploeg, et al., “Antigenic Variation in Trypanosoma Brucei Analyzed by Electrophoretic Separation of Chromsome-Sized DNA Molecules,” Cell, vol. 37, May 1984, pp. 77-84 (8 pages).
McPeek, et al., “Separation of Large DNA Molecules by Modified Pulsed Field Gradient Gel Electrophoresis,” Analytical Bioychemistry, (1986) 156, pp. 274-285 (12 pages).
Borst, et al., “Structure of Amplified DNA, Analyzed by Pulsed Field Gradient Gel Electrophoresis,” Journal of Cellular Biochemistry, (1987) 34, pp. 247-258 (12 pages).
Riveron, et al., “Un prototipo para la electroforesis de campo pulsante. Estandarización de las condiciones para la resolución de grandes moléculas de ADN,” Interferón y Biotecnologia, vol. 5, No. 1, (1988) pp. 66-76 (6 pages) (including English summary).
Dawkins, “Large DNA Separation Using Field Alternation AGAR Gel Electrophoresis,” Journal of Chromatography, 492 (1989) pp. 615-639 (25 pages).
Akerman, et al., “Reorientational Dynamics and Mobility of DNA During Pulsed-Field Agarose Gel Electrophoresis,” Journal of Physical Chemistry, (1990), 94, pp. 3828-3838 (11 pages).
Norden, et al., “Electrophoretic Orientation of DNA,” Int. Conf. El

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method and apparatus for generating electric fields and flow... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and apparatus for generating electric fields and flow..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for generating electric fields and flow... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4064164

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.