Method and apparatus for fringe-scanning chromosome analysis

Image analysis – Histogram processing – For setting a threshold

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356336, 356338, 377 10, G06K 900, G01N 1502

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active

045960364

ABSTRACT:
Apparatus and method are provided for analyzing sub-micron-sized features of microscopic particles. Two central features of the invention are (1) constraining microscopic particles to flow with substantially constant orientation through a predetermined interference fringe pattern, and (2) estimating particle structure by analyzing its fringe profile. The invention allows nearly an order of magnitude higher resolution of chromosome structure than possible with currently available flow system techniques. The invention allows rapid and accurate flow karyotyping of chromosomes.

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Norgreen, et al., "Restoration of Profiles from Slit-Scan Flow Cytometry," IEEE Transactions on Biomedical Engineering, vol. BME-29, pp. 101-106 (1982).
Carrano, et al., "Flow Cytogenetics: A New Approach to Chromosome Analysis," Flow Cytometry and Sorting, Chapter 23, John Wiley and Sons, New York.
Gray, et al., "Slit-Scan Flow Cytometry of Mammalian Chromosomes," J. Histochem. Cytochem., vol. 27, pp. 441-444 (1979).

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