Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1996-02-23
1999-03-30
Achutamurthy, Ponnathapura
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204547, 4352841, G01N 2726
Patent
active
058883703
ABSTRACT:
The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to the invention involve using the apparatus for discriminating and separating matter for research, diagnosis of a condition, and therapeutic purposes. Examples of such methods may include separation of mixtures of cells, such as cancer cells from normal cells, separation of parasitized erythrocytes from normal erythrocytes, separation of nucleic acids, and others.
REFERENCES:
patent: 4001102 (1977-01-01), Batha et al.
patent: 4326934 (1982-04-01), Pohl
patent: 4440638 (1984-04-01), Judt et al.
patent: 5344535 (1994-09-01), Betts et al.
patent: 5454472 (1995-10-01), Benecke et al.
patent: 5489506 (1996-02-01), Crane
patent: 5569367 (1996-10-01), Betts et al.
Zhang et al., "Suppressed Transformation and Induced Differentiation of HER-2/Neu Overexpressing Breast Cancer Cells by Emodin," Cancer Res., 55:3890-3896, 1995.
Jinsart et al., "Inhibition of Myosin Light Chain Kinase, cAMP-Dependent Protein Kinase, Protein Kinase C and of Plant CA-Dependent Protein Kinase by Anthraquinones," Biological Chemistry, 373:903-910, 1992.
Yeh et al., "Effects of Anthraquinones of Polygonum cuspidatum on HL-60 Cells," Planta Medica, 54:413-414, 1988.
Zhang et al., "Sensitization of HER-2/Neu Overexpressing Non-Small Cell Lung Cancer Cells to Chemotherapeutic Drugs By Tyrosine Kinase Inhibitor Emodin," Oncogene, 12:571-576, 1996.
Davis et al., "Feasibility Study of Dielectrical Field-Flow Fractionation," Separation Science and Technology, 21(9):969-989, 1986.
Arnold and Zimmerman, "Rotation of an isolated cell in a rotating electric field," Naturwissenschaften, 69:297-300, 1982.
Becker et al., "Separation of human breast cancer cells from blood by differential dielectric affinity," Proc. Natl. Acad. Sci. USA, 92:860-864, Jan., 1995.
Becker et al., "The removal of human leukaemia cells from blood using interdigitated microelectrodes," J. Phys. D. Appl. Phys., 27:2659-2662, 1994.
Fuhr Uber die rotation dielektrischer korper in rotierenden feldern, Ph.D. Dissertation, Humboldt-Universitat, Berlin, Chap. 3, pp. 24-53, 1985. Brief summary (in English) enclosed.
Gascoyne et al., "Dielectrophoretic Separation of Cancer Cells from Blood," presented at the Institute for Electrical Engineers Industrial Application Society meeting, Orlando, Florida, Oct., 1995.
Gascoyne et al., "Dielectrophoretic separation of mammalian cells studied by computerized image analysis," Meas. Sci. Technol., 3:439-445, 1992.
Gascoyne et al., "Manipulation of erythroleukemia cells using travelling electric fields," Proc. 16th IEEE-Eng. Med. Biol. Soc., 772-773, 1994.
Gascoyne et al., "Numerical analysis of the influence of experimental conditions on the accuracy of dielectric parameters derived from electrorotation measurements," Bioelectrochemistry and Bioenergetics, 36:115-125, 1995.
Gascoyne et al., "Cell Separation by Conventional Dielectrophoresis Combined with Field-Flow-Fractionation," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A333, 17-21 Feb., 1996.
Giddings, "Field-Flow Fractionation: Analysis of Macromolecular, Colloidal, and Particulate Materials," Science, 260:1456-1465, Jun., 1993.
Hagedorn et al., "Travelling-wave dielectrophoresis of microparticles," Electrophoresis, 13:49-54, 1992.
Holzel and Lamprecht, "Dielectric properties of yeast cells as determined by electrorotation," Biochim. Biophys. Acta, 1104:195-200, 1992.
Huang et al., "Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies," Phys. Med. Biol., 37:1499-1517, 1992.
Huang et al., "Application of AC Electrokinetics for Cell Characterization and Manipulation," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A334, 17-21 Feb., 1996.
Huang et al., "Electrorotational studies of the cytoplasmic dielectric properties of Friend murine erythroleukaemia cells," Phys. Med. Biol., 40:1789-1806, 1995.
Huang et al., "Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells," J. Phys. D. Appl. Phys., 26:1528-1535, 1993.
Markx et al., "Dielectrophoretic characterization and separation of micro-organisms," Microbiol., 140:585-591, 1994.
Markx and Pethig, "Dielectrophoretic Separation of Cells: Continuous Separation," Biotechnology and Bioenineering, 45:337-343, 1995.
Wang et al., "A unified theory of dielectrophoresis and travelling wave dielectrophoresis," J. Phys. D. Appl. Phys., 27:1571-1574, 1994.
Wang et al., "Dielectrophoretic Manipulation of Cells Using Sprial Electrode Arrays," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A333, 17-21 Feb., 1996.
Wang et al., "Dielectrophoretic Manipulation of Particles," presented at The Institute for Electrical Engineers Industrial Application Society meeting, Orlando, Florida, Oct., 1995.
Wang et al., "Non-uniform spatial distributions of both the magnitude and phase of AC electric fields determine dielectrophoretic forces," Biochimica et. Biophysica Acta, 1243:185-194, 1995.
Wang et al., "Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation," Biochimica et Biophysica Acta, 1193:330-334, 1994.
Washizu et al., "Molecular Dielectrophoresis of Biopolymers," IEEE Trans on Industry App, 30(4):835-843, Aug. 1994.
B.S. Massey, "Mechanics of Fluids, 2nd Edition" (1975) 136-139.
John K. Vennard, "Elementary Fluid Mechanics" (1954) 150-155.
Becker Frederick F.
Gascoyne Peter R. C.
Huang Ying
Wang Xiao-Bo
Achutamurthy Ponnathapura
Board of Regents , The University of Texas System
Ponnaluri P.
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