Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1996-01-31
1999-11-30
Till, Terrence R.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204643, G01N 2726, G01N 27447
Patent
active
059936304
ABSTRACT:
The present disclosure is directed to a novel apparatus and novel methods for the separtion, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to the invention involve using the apparatus for discriminating and separating matter for research, diagnosis of a condition, and therapeutic purposes. Examples of such methods may include separation of mixtures of cells, such as cancer cells from normal cells, separation of parasitized erythrocytes from normal erythrocytes, separation of nucleic acids, and others.
REFERENCES:
patent: 4001102 (1997-01-01), Batha et al.
patent: 4326934 (1982-04-01), Pohl
patent: 4440638 (1984-04-01), Judy et al.
patent: 5344535 (1994-09-01), Betts et al.
patent: 5454472 (1995-10-01), Benecke et al.
patent: 5489506 (1996-02-01), Crane
patent: 5569367 (1996-10-01), Betts et al.
B.S. Massey, "Mechanics of Fluids, 2nd edition" (1975) 136-139.
John K. Vennard, "Elementary Fluid Mechanics" (1954) 150-155.
Joe M. Davis and J. Calvin Giddings, "Feasibility Study of Dielectrical Field-Flow Fractionation" Separation Science and Technology, 21(9) [1986*] 969-989.
Washizu et al., "Molecular Dielectrophoresis of Biopolymers," IEEE Trans on Industry App, 30(4):835-843, Aug. 1994.
Zhang et al., "Suppressed Transformation and Induced Differentiation of HER-2/Neu Overexpressing Breast Cancer Cells By Emodin," Cancer Res., 55:3890-3896, 1995.
Jinsart et al., "Inhibition of Myosin Light Chain Kinase, cAMP-Dependent Protein Kinase, Protein Kinase C and of Plant CA-Dependent Protein Kinase by Anthraquinones," Biological Chemistry, 373:903-910, 1992.
Yeh et al., "Effects of Anthraquinones of Polygonum cuspidatum on HL-60 Cells," Planta Medica, 54:413-414, 1988.
Zhang et al., "Sensitization of HER-2/Neu Overexpressing Non-Small Cell Lung Cancer Cells to Chemotherapeutic Drugs By Tyrosine Kinase Inhibitor Emodin," Oncogene, 12:571-576, 1996.
International Search Report dated Jun. 9, 1997 (UTFC: 527P).
Arnold and Zimmermann, "Rotation of an isolated cell in a rotating electric field," Naturwissenschaften, 69:297-300, No month available 1982.
Becker et al., "Separation of human breast cancer cells from blood by differential dielectric affinity," Proc. Natl. Acad. Sci. USA, 92:860-864, Jan., 1995.
Becker et al., "The removal of human leukaemia cells from blood using interdigitated microelectrodes," J. Phys. D. Appl. Phys., 27:2659-2662, No month available 1994.
Fuhr, Uber die rotation dielectrischer korper in rotierenden feldern, Ph.D. Dissertation, Humboldt-Universitat, Berlin, Chap. 3, pp. 24-53, No month available 1985.
Gascoyne et al., "Dielectrophoretic Separation of Cancer Cells from Blood," presented at the Institute for Electrical Engineers Industrial Application Society meeting, Orlando, Florida, Oct., No month available 1995.
Gascoyne et al., "Dielectrophoretic separation of mammalian cells studied by computerized image analysis," Meas. Sci. Technol., 3:349-445, No month available 1992.
Gascoyne et al., "Manipulation of erythroleukemia cells using travelling electric fields," Proc. 16th IEEE-Eng. Med. Biol. Soc., 772-773, No month available 1994.
Gascoyne et al., "Numerical analysis of the influence of experimental conditions on the accuracy of dielectric parameters derived from electrorotation measurements," Bioelectrochemistry and Bioenergetics, 36:115-125, No month available 1995.
Gascoyne et al., "Cell Separation by Conventional Dielectrophoresis Combined with Field-Flow-Fractionation," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A333, Feb. 17-21, No month available 1996.
Giddings, "Field-Flow Fractionation: Analysis of Macromolecular, Colloidal, and Particulate Materials," Science, 260:1456-1465, Jun., 1993.
Hagedorn et al., "Travelling-wave dielectrophoresis of microparticles," Electrophoresis, 13:49-54, 1992.
Holzel and Lamprecht, "Dielectric properties of yeast cells as determined by electrorotation," Biochim. Biophys. Acta, 1104:195-200, No month available 1992.
Huang et al., "Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies," Phys. Med. Biol., 37:1499-1517, No month available 1992.
Huang et al., "Application of AC Electrokinetics for Cell Characterization and Manipulation," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A334, Feb. 17-21, 1996.
Huang et al., "Electrorotational studies of the cytoplasmic dielectric properties of Friend murine erythroleukaemia cells," Phys. Med. Biol., 40:1789-1806, No month available 1995.
Huang et al., "Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells," J. Phys. D. Appl. Phys., 26:1528-1535, no month available 1993.
Markx et al., "Dielectrophoretic characterization and separation of micro-organisms," Microbiol., 140:585-591, No month available 1994.
Markx and Pethig, "Dielectrophoretic Separation of Cells: Continuous Separation," Biotechnology and Bioengineering, 45:337-343, No month available 1995.
Wang et al., "A unified theory of dielectrophoresis and travelling wave dielectrophoresis," J. Phys. D. Appl. Phys., 27:1571-1574, No month available 1994.
Wang et al., "Dielectrophoretic Manipulation of Cells Using Spiral Electrode Arrays," Abstract, 40th Annual Meeting, Baltimore, Maryland, p. A333, Feb. 17-21, 1996.
Wang et al., "Dielectrophoretic Manipulation of Particles," presented at The Institute for Electrical Engineers Industrial Application Society meeting, Orlando, Florida, Oct., 1995.
Wang et al., "Non-uniform spatial distributions of both the magnitude and phase of AC electric fields determine dielectrophoretic forces," Biochimica et. Biophysica Acta, 1243:185-194, No month available 1995.
Wang et al., "Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation," Biochimica et Biophysica Acta, 1193:330-334, No month available 1994.
Becker Frederick F.
Gascoyne Peter R. C.
Huang Ying
Wang Xiao-Bo
Board of Regents , The University of Texas System
Starsiak Jr. John S.
Till Terrence R.
LandOfFree
Method and apparatus for fractionation using conventional dielec does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and apparatus for fractionation using conventional dielec, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for fractionation using conventional dielec will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1667080